한국농림기상학회지, 제 10권 제3호(2008) (pISSN 1229-5671, eISSN 2288-1859)
Korean Journal of Agricultural and Forest Meteorology, Vol. 10, No. 3, (2008), pp. 75~81
DOI: 10.5532/KJAFM.2008.10.3.075
ⓒ Author(s) 2014. CC Attribution 3.0 License.


인공산성비 처리에 의한 백합나무 묘목의 엽피해와 엽록소함량 변화

윤준혁(1), 이도형(1), 우관수(2)
(1)영남대학교 산림자원학과, (2)국립산림과학원 산림유전자원부

(2008년 06월 18일 접수; 2008년 09월 24일 수정; 2008년 09월 26일 수락)

Changes in Chlorophyll Content and Leaf Damages of
Liliodendron tulipifera L. Seedlings Treated with
Simulated Acid Rain

Jun-Hyuck Yoon(1), Do-Hyung Lee(1), Kwan-Soo Woo(2)
(1)Department of Forest Resources, Yeungnam University, Gyeongsan 712-749, Korea
(2)Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon 441-847, Korea

(Received June 18, 2008; Revised September 24, 2008; Accepted September 26, 2008)

ABSTRACT
This study was conducted to analyze the influence of simulated acid rain on leaves of Liliodendron tulipifera seedlings. The seedlings were treated with four levels of simulated acid rain with pH of 5.6, 4.9, 3.9 and 2.9, and then chlorophyll contents and the degree of foliar damage were investigated. Differences were statistically significant among treatments in chlorophyll contents in all of the tested soils. The total contents of chlorophyll tended to decrease as the simulated acid rain treatment was maintained. Chlorophyll contents, however, did not decrease according to the decrement of pH levels. Foliar damage increased as the simulated acid rain was maintained in all tested soils. Especially, the damage dramatically increased between May and July as the acidity increased. As the treatment of simulated acid rain continued, deformed new leaves appeared. In all treatments except the control, the leaves turned brown and the damage increased with time. Necrotic spots appeared during the first month of treatment at the pH level of 2.9 in all soil types. The damage by acid rain should be considered when we plant Liliodendron tulipifera.

Keyword: Liliodendron tulipifera, Simulated acid rain, Chlorophyll content, Foliar damage

MAIN

적요

인공산성비 처리가 백합나무 묘목의 잎에 미치는 영향을 분석하기 위하여 인공적으로 pH 5.6, 4.9, 3.9, 2.9로 구분된 산성비를 일정기간 처리하여 엽록소 함량, 엽피해율 및 가시적 피해증상을 조사한 결과, 엽록소 함량의 경우, pH 수준 간 통계적으로 유의미한 차이가 나타났으나 pH 수준별 순차적 감소는 나타나지 않았고 인공산성비 처리가 지속됨에 따른 엽록소함량의 감소를 보였다. 또한, 엽록소 함량은 세 가지 토양에서 모두 pH 2.9처리구에서 가장 큰 폭으로 감소하였다. 엽피해율은 인공산성비 처리 초기시점에 급격한 피해를 보이다가 지속적으로 피해가 나타났다. 또한, pH 수준이 낮아짐으로써 엽피해율도 차이를 나타내었으며, 가시적 피해증상으로는 기형잎, 갈변현상, 괴사반점 형태로 나타났다. 본 연구의 결과를 전반적으로 고려할 때, 인공산성비 처리는 백합나무 묘목의 잎에서 일어나는 광합성작용 및 물리적 피해를 준 것으로 나타났다. 따라서 최근 가로수 및 용재수로 보급 중에 있는 백합나무에 대하여 산성비에 의해 예상되는 내외부 생리적 반응 및 생장에 미치는 영향 등에 관한 체계적이고 부가적인 연구가 요구된다.

REFERENCES

Arnon, D. I., 1959: Copper enzyme in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology 24, 1-15

Cape, J. N., 1986: Effects of air pollution on chemistry of surface waxes of scots pines. Water, Air and Soil Pollution 31, 393-399crossref(new window)

Cheong, Y. M., 1987: Simulated acid rain on growth and nutrient contents of Pinus densiflora S. et Z. and Forsythia koreana nak. seedlings and on chemical properties of the experimental soil. Ph. D. Dissertation. Dongguk University, 70pp.

Cheong, Y. M., 1988: Effect of simulated acid rain on nutrient contents of Pinus densiflora S. et Z. and Forsythia koreana Nak. Seedlings. Journal of Korea Forest Society 77(3), 259-268. (in Korean with English abstract)

Cole, D. W., and D. W. Johnson, 1977: Atmospheric sulfate additions and cation leaching in a Douglas fir ecosystem. Water Resource Research 392, 313-317

Evans, L. S., 1980: Foliar responses that may determine plant injury by simulated acid rain. Polluted Rain, T.Y. Yoribara, M.W. Miller and P.E. Morow (Eds). Plenum Press, New York, pp. 239-254

Evans, L. S., 1984: Botanical aspects of acidic precipitation. Botanical Review 50, 449-490crossref(new window)

Ferenbaugh, R. W., 1976: Effects of simulated acid rain on Phaseolus vulgaris L. (Fabaceae). American Journal of Botany 63(3), 283-288crossref(new window)

Garsed, S. G., and R. A. Rutter, 1982: Relative performance of conifer populations in various tests for sensitivity to $SO_2$, and the implications for selecting trees for planting in polluted areas. New Phytologist 92, 349-367crossref(new window)

Han, S. H., and K. J. Lee, 1997: Buffering capacity of four tree species against soil acidification by acid rain and variations in nutrient leaching from tree crowns. Journal of Korea Forest Society 86(3), 342-351. (in Korean with English abstract)

Hindawi, I. J., J. A. Rea, and W. L. Griffis, 1980: Response of bush bean exposed to acid mist. American Journal of Botany 67, 168-172crossref(new window)

Kelly, J. M., and R. C. Strickland, 1986: Throughfall and plant nutrient concentration response to simulated acid rain treatment. Water, Air and Soil pollution 29, 219-231crossref(new window)

Kim, C. H., and Y. M. Cheong, 1987: Effect of simulated acid rain on the chlorophyll contents in the needles of Pinus koraiensis and Ligustrum obtusifolium seedlings. Journal of Plant Biology 76(1), 11-16. (in Korean with English abstract)

Kim, M. R., and W. Y. Soh, 1994: Growth response of Ginkgo biloba and Pinus thunbergii exposed on simulated acid rain. Journal of Plant Biology 37(1), 93-99. (in Korean with English abstract)

Kim, M. R., and W. Y. Soh, 1995: Effect of simulated acid rain on foliar structure of changes of Ginko biloba and Pinus thunbergii. Journal of Plant Biology 38(1), 79-86. (in Korean with English abstract)

Kim, G. T., 1987: Effects of simulated acid rain on growth and physiological characteristics of Ginkgo biloba L. seedlings and on chemical properties of the tested soil. Ph. D. Dissertation. Seoul National University, 46pp

Kim, G. T., 1992: Effects of artificial acid rain on seed germination, radicle growth and seedling growth of several woody species. Journal of Korea Forest Society 81(1), 30-39. (in Korean with English abstract)

Korea Forest Research Institute, 2004: Characteristics and forestation skill on Liliodendron tulipifera (II), 8pp

Lee, J. J., and D. E. Weber, 1979: The effect of simulated acid rain on seedling emergence and growth of eleven woody species. Forest Science 2, 393-398

Lee, J. J., and D. E. Weber, 1982: Effects of sulfuric acid rain on major cation and sulfate concentrations of water percolating through two model hardwood forests. Journal of Environmental Quality 11, 57-64

McKinney, G., 1941: Absorption of light by chlorophyll solutions. Journal of Biological Chemistry 140, 315-322

Oh, J. H., 1986: Effect of simulated acid rain on the growth of trees and soil. M. S. Thesis. Kyung Hee University, 28pp

Percy, K. E., and E. A. Baker, 1987: Effects of simulated acid rain on production, morphology and composition of epiculticular wax and on culticular membrane development. New Phytologist 107, 577-589crossref(new window)

Schier, G.A., 1990: Response of yellow-poplar (Liriodendron tulipifera L.) seedlings to simulated acid rain and ozone- 2. Effect on throughfall chemistry and nutrients in the leaves. Environmental and Experimental Botany 30(3), 325-331crossref(new window)