한국농림기상학회지, 제 10권 제2호(2008) (pISSN 1229-5671, eISSN 2288-1859)
Korean Journal of Agricultural and Forest Meteorology, Vol. 10, No. 2, (2008), pp. 47~57
DOI: 10.5532/KJAFM.2008.10.2.047
ⓒ Author(s) 2014. CC Attribution 3.0 License.


참나무속 5종의 오존 독성에 대한 생리생화학적 반응

김두현(1), 한심희(1), 구자정(1), 이갑연(1), 김판기(2)
(1)국립산림과학원 산림유전자원부, (2)경북대학교 산림환경자원학부

(2008년 05월 07일 접수; 2008년 06월 13일 수정; 2008년 06월 25일 수락)

Physiological and Biochemical Responses to Ozone Toxicity in
Five Species of genus Quercus Seedlings

Du-Hyun Kim(1), Sim-Hee Han(1), Ja-Jung Ku(1), Kab-Yeon Lee(1), Pan-Gi Kim(2)
(1)Divison of Forest Genetic Resources, Korea Forest Research Institute, Suwon, 441-350, Korea
(2)Division of Forest Environment and Resources, Kyungpook National University, Sangju, 742-711, Korea

(Received May 07, 2008; Revised June 13, 2008; Accepted June 25, 2008)

ABSTRACT
Physiological and biochemical changes of five species of genus Quercus exposed to ozone fumigation were investigated to assess their tolerance against ozone toxicity. At the end of 150 ppb ozone fumigation, chlorophyll contents, photosynthetic characteristics, malondialdehyde(MDA) and antioxidative enzyme activities were measured in the leaves of five Quercus species(Quercus acutissima, Q. aliena, Q. palustris, Q. serrata, and Q. variabilis). Chlorophyll and carotenoid contents, net photosynthesis and carboxylation efficiency decreased after ozone treatment, indicating that O3-exposed plants underwent physiological inhibition. The reduction rate of total chlorophyll contents and carboxylation efficiency were respectively 15% and 34% for Q. aliena and 38% and 62% for Q. variabilis. The amount of MDA increased with the highest increase rate of 140% in Q. acutissima which also showed the highest increase rate(60%) of superoxide dismutase(SOD). Ascorbate peroxidase(APX) activity increased in Q. variabilis, Q. serrata and Q. acutissima by ozone treatment. Based on our results, ozone tolerance of the five Quercus species was ranked as Q. aliena>Q. palustris>Q. serrata>Q. variabilis>Q. acutissima. We concluded that chlorophyll contents, photosynthesis, MDA content and antioxidative enzymes were the important physiological markers for tolerance against ozone stress, which were closely related with one another.

Keyword: Oak, Chlorophyll content, Photosynthesis, MDA, Antioxidative enzyme

MAIN

적요

오존에 노출된 참나무속 5종의 오존에 대한 내성 능력을 평가하기 위하여 생리생화학적 변화를 조사하였다. 150ppb 오존에 노출된 참나무속 5종(상수리나무,갈참나무, 대왕참나무, 졸참나무, 굴참나무)의 잎에서 엽록소 함량, 광합성 특성, MDA 함량 및 항산화효소 활성이 측정되었다. 엽록소, 카로테노이드 함량, 순광합성 속도 및 탄소고정효율은 오존 처리 후에 감소하였다. 오존에 노출된 수목의 총 엽록소 함량과 탄소고정효율의 감소율은 갈참나무의 경우 15%와 34% 였으며, 굴참나무의 경우 38.3%와 62.1%였다. MDA 함량은 오존 처리 하에서 증가하였으며, 상수리나무에서 140%까지 증가를 보였다. 상수리나무의 SOD 활성 증가율(60%)은 가장 높았으며, APX 활성은 굴참나무, 졸참나무, 상수리나무에서 증가를 보였다. 생리생화학적 반응을 기초로 한 참나무속 5종의 내성 능력은 갈참나무, 대왕참나무, 졸참나무, 굴참나무, 상수리나무순이었다. 결론적으로 엽록소 함량, 광합성 특성, MDA 함량, 항산화효소와 같은 생리학적 지표들은 오존 스트레스에 대한 내성을 평가하기 위한 매우 중요한 지표들로 생각되며, 이러한 모수들은 서로 밀접한 관계를 가진다.

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