한국농림기상학회지, 제 11권 제1호(2009) (pISSN 1229-5671, eISSN 2288-1859)
Korean Journal of Agricultural and Forest Meteorology, Vol. 11, No. 1, (2009), pp. 3~12
DOI: 10.5532/KJAFM.2009.11.1.003
ⓒ Author(s) 2014. CC Attribution 3.0 License.


표준화 지수를 이용한 환경수목의 오존 내성 결정

한심희(1), 김두현(1)
(1)국립산림과학원 산림유전자원부

(2009년 03월 04일 접수; 2009년 03월 13일 수정; 2009년 03월 19일 수락)

Determination of Ozone Tolerance on Environmental
Tree Species Using Standard Index

Sim-Hee Han(1), Du-Hyun Kim(1)
(1)Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon 441-350, Korea

(Received March 04, 2009; Revised March 13, 2009; Accepted March 19, 2009)

ABSTRACT
Ozone tolerance of tree species was determined by standard index of physiological damages and biochemical defense responses under short-term ozone exposure. At the end of 150ppb O3 fumigation, photosynthetic characteristics and antioxidative enzyme activities were analyzed in the leaves of five species(Koelreuteria paniculata, Firmiana simplex, Styrax japonica, Fraxinus rhynchophylla, Viburnum sargentii). Injury index was determined by the effect of ozone on photosynthetic parameters and malondialdehyde(MDA) content, and tolerance index was calculated using the rate of increase in superoxide dismutase(SOD), ascorbate-peroxidase(APX), glutathione reductase(GR) and catalase(CAT) activities. Apparent quantum yield(AQY), carboxylation efficiency(Ce) and photo-respiration rate(PR) decreased in the leaves of five species with increasing ozone exposure time. These parameters were considered as an appropriate indicator for stress evaluation. Antioxidative enzyme activities showed various results depending on the tree species, exposure time, and enzyme types. SOD activity of K. paniculata increased with ozone exposure time, and that of F. rhynchophylla increased only after 6 hours of ozone exposure. CAT activity of O3-exposed F. simplex was lower than the control. Based on standard index, ozone tolerance ability of five species was determined as two tolerant species(F. rhynchophylla > K. paniculata) and three sensitive species(S. japonica > F. simplex > V. sargentii).

Keyword: Physiological damage, Antioxidative enzyme, Photosynthetic parameters, Standard index

MAIN

적요

수목의 오존 내성을 단기 노출 후 나타난 생리적 피해와 생화학적 반응들의 표준화 지수로 결정하였다. 모감주나무, 벽오동, 때죽나무, 물푸레나무, 백당나무를 150ppb 오존 노출 시킨 후, 광합성 특성, MDA 함량 및 항산화효소의 활성을 측정하여 피해지수는 광합성 파라미터와 MDA 함량 변화를 이용하여 계산하였으며, 내성지수는 항산화효소의 활성 변화를 측정하여 산출하였다. 순양자수율, 탄소고정효율, 광호흡속도는 오존노출시간에 따라 감소하여, 스트레스 평가지표로 적당한 것으로 판단되었다. 항산화효소의 활성은 수종, 노출시간 및 효소 종류에 따라 다양한 결과를 보여 주었다. 모감주나무 SOD 활성은 오존 노출시간에 따라 증가하였고, 물푸레나무의 SOD 활성은 오존 노출 6시간 후에 증가하였으며, 벽오동의 CAT 활성은 무처리보다 낮았다. 표준화 지수를 기초로 한 5개 수목의 오존 내성은 두 내성 수종(물푸레나무>모감주나무)과세 민감성 수종(때죽나무>벽오동>백당나무)으로 구분되었다.

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