한국농림기상학회지, 제 5권 제3호(2003) (pISSN 1229-5671, eISSN 2288-1859)
Korean Journal of Agricultural and Forest Meteorology, Vol. 5, No. 3, (2003), pp. 172~178
ⓒ Author(s) 2014. CC Attribution 3.0 License.


탄광 폐석지내 자생 수종의 생리적 피해 및 내성

이재천(1), 한심희(1), 장석성(1), 김판기(2), 허재선(3), 염규진(4)
(1)임업연구원, (2)서울대학교, (3)순천대학교, (4)(주)코엔바이오

(2003년 07월 10일 접수; 2003년 08월 06일 수락)

Physiological Tolerance of Native Tree Species
in Abandoned Coal Mine Spoils

Jae-Cheon Lee(1), Sim-Hee Han(1), Suk-Seong Jang(1), Pan-Gi Kim(2), Jae-Seoun Hur(3), Kyu-Jin Yum(4)
(1)Korea Forest Research Institute, Suwon 441-350, Korea
(2)Seoul National University, Seoul 151-742, Korea
(3)SunChon National University, SunChon 540-742, Korea
(4)Coenbio Co. Ltd., Suwon 445-743, Korea

(Received July 10, 2003; Accepted August 06, 2003)

ABSTRACT
This study was conducted to assess the physiological tolerance of native tree species for successful restoration and revegetation of abandoned coal-mine spoils. Study sites were two coal-mine spoils (Sododong and Ssarijae) in Taebaek, Kangwon Province, Korea. Five individuals of Betula costata and of B. schmidtii were analyzed for malondialdehyde (MDA) and hydrogen peroxide ($H_2O$ 수식 이미지$_2$ 수식 이미지) content, nitrate reductase (NR) and superoxide dismutase (SOD) activity, and for carbohydrate concentration in the leaves. Trees in the abandoned coal-mine spoils were influenced by deficiencies expressed by MDA and $H_2O$ 수식 이미지$_2$ 수식 이미지 content in the leaves of two species being higher at the coal-mine spoils than in the surrounding forest. Low NR activity indirectly represented nitrogen deficiency in the soil of the coal-mine spoils; an unmanageable SOD activity implied that tolerant functions didn’t net against a certain stress of the coal-mine spoils. Decreased glucose and increased starch concentration especially showed the inhibition of the carbohydrate metabolism by inadequate factors. Consequently, low nitrogen content in the real-mine soils might increase damage in trees as a result of inhibiting the expression of tolerance mechanisms against stress. Therefore, trees in coal-mine spoils need ample nitrogen to use as a metabolic energy source in order to prevent damage and increase tolerance against stress.

Keyword: coal mine spoil, Betula, restoration, revegetation, MDA, H2O2, NR, SOD, carbohydrate

MAIN

적요

본 연구는 폐석지의 성공적인 식생 복원을 위하여 자생 수종을 대상으로 피해 수준을 평가하고, 내성 특성을 구명하고자 실시하였다. 연구 조사지는 강원도 태백시 소도동과 싸리재에 위치한 폐석지를 대상으로 하였으며, 이미 선발한 거제수나무와 박달나무의 잎을 채취하여 NR 활성, MDA와 $H_2O$ 수식 이미지$_2$ 수식 이미지 함량, SOD 활성, 탄수화물 함량을 분석하였다. 폐석지 내 수목들은 폐석지 주변 산림 내 수목들 보다 MDA함량과 $H_2O$ 수식 이미지$_2$ 수식 이미지 함량이 높아 폐석지 내 부적당한 환경 요인에 영향을 받고 있음을 보여 주었다. 또한 낮은 NR 활성은 폐석지 내 질소원의 부족을 간접적으로 나타냈으며, 폐석지내 수목의 잎에서 항산화 효소인 SOD의 활성 증가가 나타나지 않은 것으로 보아 스트레스에 대한 내성 기능이 적절하게 작용하지 못하고 있음을 알 수 있었다. 특히 포도당의 감소와 전분의 증가는 탄수화물 대사가 부적당한 환경 요인에 의해 저해되고 있음을 보여 주었다. 결론적으로 폐석지 토양 내 낮은 질소함량은 스트레스에 대한 수목의 내성 발현에 도움을 주지 못하여 수목의 피해가 증가한 것으로 판단된다. 따라서 폐석지 내 수목의 피해를 막고 스트레스에 대한 내성을 증가시키기 위해서는 각종 대사의 에너지원으로 이용되는 양료 공급이 절실히 요구된다.

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