한국농림기상학회지, 제 8권 제2호(2006) (pISSN 1229-5671, eISSN 2288-1859)
Korean Journal of Agricultural and Forest Meteorology, Vol. 8, No. 2, (2006), pp. 77~85
ⓒ Author(s) 2014. CC Attribution 3.0 License.


토양수분과 관수량이 곤충병원성선충의 병원성에 미치는 영향
이동운(1), 최우근(2), 이상명(3), 김형환(4), 추호렬(5)
(1)상주대학교 생물응용학과, (2)서원벨리골프장, (3)국립산림과학원 남부산림연구소,
(4)원예연구소 원예환경과, (5)경상대학교 응용생물환경학과, 농업생명과학연구원

(2006년 04월 19일 접수; 2006년 05월 25일 수락)

Effect of Soil Moisture and Irrigation on Pathogenicity of
Entomopathogenic Nematodes
Dong Woon Lee(1), Woo Geun Choi(2), Sang Myeong Lee(3), Hyeong Hwan Kim(4), Ho Yul Choo(5)
(1)Department of Applied Biology, Sangju National University, Sangju, Gyeongbuk, 742-711, Republic of Korea
(2)Seowon Valley Golf Club, Paju, Gyeonggii, 413-852, Republic of Korea
(3)Southern Forest Research Center, National Forest Science Institute, Jinju,
Gyeongnam, 660-300, Republic of Korea
(4)Horticultural Environment Division of national Horticultural Research Institute, RDA.
Suwon, 441-440, Republic of Korea
(5)Department of Applied Biology and Environmental Sciences, Institute of Agriculture & Life Sciences,
Gyeongsang National University, Jinju, Gyeongnam, 660-701, Republic of Korea.

(Received April 19, 2006; Accepted May 25, 2006)

ABSTRACT
Entomopathogenic nematodes (EPNs) have been used as biological control agents for control of various agro-forest insect pests, and are especially effective against soil-dwelling insect pests. Effect of soil moisture on pathogenicity of commercial EPNs for white grub control was evaluated in laboratory, pots, and golf courses. Pathogenicity of EPNs in sand column was variable depending on depth, soil moisture, and EPN species or strain. All tested EPNs (Heterorhabditis sp. GSNUH1, Heterorhabditis sp. GSNUH2, Steinernema carpocapsae GSN1, and S. longicaudum Nonsan strain) showed similar pathogenicity against the bait insect, great wax moth (Galleria mellonella) larva at 2 cm deep at a given soil moisture. However, pathogenicity of the Heterorhabditis sp. GSNUH1 strain was decreased with increasing soil moisture. Pathogenicity of S. carpocapsae GSN1 strain was the lowest in 3% soil moisture (v/w) at 7 cm depth. However, there was no difference in pathogenicity between Heterorhabditis sp. GSNUH2 and S. longicaudum Nonsan strain. Although pathogenicity of Heterorhabditis sp. KCTC 0991BP strain showed no difference against the 2nd instar of Exomala orientalis, that of the S. carpocapsae GSN1 strain was decreased in the laboratory depending on soil moisture. Highly pathogenic strain EPN, Heterorhabditis sp. KCTC 0991BP strain, showed higher pathogenicity at 100 mm irrigation than non-irrigation or 10 mm irrigation. However, poor pathogenic strain EPN, S. carpocapsae GSN1 strain, was not different in pathogenicity from the 2nd instar of Exomala orientalis in creeping bentgrass (Agrostis palustris) depending on irrigation amount in the pot. Pathogenicity of EPNs in field experiment at the tee of Ulsan golf club showed a similar trend to that in the pot experiment.

Keyword: Entomopathogenic nematode, Pathogenicity, Soil moisture, Irrigation, White grub

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